Coding

Part:BBa_K200015:Design

Designed by: Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-14)

Coding region for the DpnII restriction enzyme.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NOTE: Unlike other restriction enzymes that show Star Activity at high pH 8.0, DpnII has Star Activity above approximately pH 6.5. The effect of pH on Star Activity of DpnII is very extreme, showing one-thousand-fold more Star Activity at pH 7.5 than at pH 6.0.


Source

Synthesised from the genomic sequence of Streptococcus pneumoniae Hungary19A-6.

References